Molecular cloning of the β‐subunit of the Na,K‐ATPase in the brine shrimp, Artemia

FEBS Letters

Hokin, Lowell E.¹  Bergstrom, Ellen E.¹  Bhattacharyya, Kalyan K.¹ 

[1] Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706, USA

关键词: Na;    K-ATPase;    β-Subunit;    cDNA cloning;    Nucleotide sequence;    Amino acid sequence;    Artemia;   

DOI  :  10.1016/0014-5793(90)81162-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding the β-subunit of the Na,K-ATPase of brine shrimp (Artemia) has been cloned. Its nucleotide sequence and predicted amino acid sequence have been determined. The amino acid sequence shows considerable divergence from that of chicken, dog, human, pig, rat, sheep, Torpedo, and Xenopus. This is not entirely unexpected since brine shrimp is a ‘fast clock’ organism which diverged from the precursor of the vertebrates 0.5–1.0 billion years ago. However, a highly hydrophobic putative transmembrane domain and the carboxy-terminal domain show considerable conservation. The relatively small degree of conservation in the β-subunit of Anemia should provide information about the functional significance of this protein.

【 授权许可】

Unknown