Identification of a novel gene of the X,K‐ATPase β‐subunit family that is predominantly expressed in skeletal and heart muscles

FEBS Letters

Pestov, Nikolay B.²  Adams, Gail²  Shakhparonov, Mikhail I.¹  Modyanov, Nikolai N.² 

[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117871, Russia;Department of Pharmacology, Medical College of Ohio, Toledo, OH 43614, USA

关键词: P-ATPase;    Na;    K-ATPase;    H;    K-ATPase;    β-Subunit;    Tissue-specific expression;    Muscle-specific isoform;    α1;    α2;    α3;    α4;    α1-;    α2-;    α3- and α4-subunits of Na;    K-ATPase;    β1;    β2;    β3;    β1-;    β2- and β3-subunits of Na;    K-ATPase;    αg and βg;    α- and β-subunits of the gastric H;    K-ATPase;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    RT-PCR;    reverse transcription-polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;    UTR;    untranslated region;   

DOI  :  10.1016/S0014-5793(99)00954-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified the fifth member of the mammalian X,K-ATPase β-subunit gene family. The human and rat genes are largely expressed in skeletal muscle and at a lower level in heart. The deduced human and rat proteins designated as β_muscle (β_m) consist of 357 and 356 amino acid residues, respectively, and exhibit 89% identity. The sequence homology of β_m proteins with known Na,K- and H,K-ATPase β-subunits are 30.5–39.4%. Unlike other β-subunits, putative β_m proteins have large N-terminal cytoplasmic domains containing long Glu-rich sequences. The data obtained indicate the existence of hitherto unknown X,K-ATPase (most probably Na,K-ATPase) isozymes in muscle cells.

【 授权许可】

Unknown