A delayed rectifier potassium channel cloned from bovine adrenal medulla Functional analysis after expression in Xenopus oocytes and in a neuroblastoma cell line

FEBS Letters

García-Guzmán, Miguel³  Criado, Manuel³  Sala, Francisco¹  Sala, Salvador² 

[1] Department of Pharmacology, Universidad de Alicante, Ap. 374 E-03080, Alicante, Spain;Department of Physiology and Instituto de Neurociencias, Universidad de Alicante, Ap. 374 E-03080, Alicante, Spain;Department of Neurochemistry, Universidad de Alicante, Ap. 374 E-03080, Alicante, Spain

关键词: cDNA cloning;    Bovine adrenal medulla;    Potassium channel;    Stable transfection;    Heterologous expression;   

DOI  :  10.1016/0014-5793(94)01117-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Neuroblastoma cells (Neuro-2a cell line) were stably transfected with BAK5 DNA. Protein expression was under the control of a heat-shock promoter. Transfected cells showed a current highly selective for potassium, insensitive to tetraethylammonium but reversibly blocked by 4-aminopyridine. Oocytes injected with BAK5 mRNA also expressed a potassium current with the same characteristics.

【 授权许可】

Unknown