Potassium channels from NG108‐15 neuroblastoma‐glioma hybrid cells

FEBS Letters

Imoto, Keiji²  Yokoyama, Shigeru²  Miyata, Takashi¹  Iwabe, Naoyuki¹  Kawamura, Tetsuro²  Numa, Shosaku²  Higashida, Haruhiro³ 

[1] Department of Biology, Kyushu University Faculty of Science, Fukuoka 812, Japan;Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan;Department of Biophysics, Neuroinformation Research Institute, Kanazawa University School of Medicine, Kanazawa 920, Japan

关键词: Potassium channel;    cDNA cloning;    cDNA expression;    Delayed rectifier;    Phylogeny;    (NG 108-15 cell);   

DOI  :  10.1016/0014-5793(89)81488-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequences of two potassium channel proteins from NG108-15 neuroblastoma-glioma hybrid cells have been deduced by cloning and sequencing the cDNAs. One of these proteins (NGK2) is structurally more closely related to the Drosophila Shaw gene product than to the Shaker and Shab gene products, whereas the other (NGK1) is identical with a rat brain potassium channel protein (BK2) which is more closely related to the Drosophila Shaker gene product. mRNAs derived from both the cloned cDNAs, when injected into Xenopus oocytes, direct the formation of functional potassium channels with properties of delayed rectifiers.

【 授权许可】

Unknown