Changes in subcellular and zonal distribution of glucokinase in rat liver during postnatal development

FEBS Letters

Miwa, Ichitomo¹  Nonogaki, Tsunemasa²  Toyoda, Yukiyasu¹  Okuda, Jun¹  Kamiya, Masahiro¹  Ogiso, Saiko¹ 

[1] Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University, Tempaku-ku, Nagoya 468, Japan;Department of Pathology, Aichi Medical University, Nagakute-cho, Aichi 480-11, Japan

关键词: Glucokinase;    Liver;    Postnatal development;    Subcellular distribution;    Zonal distribution;    Rat;    Immunohistochemistry;   

DOI  :  10.1016/0014-5793(94)01452-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subcellular and zonal distribution of glucokinase in rat liver during postnatal development was examined immunohistochemically. Before day 11 after birth, only some hepatocytes were immunostained, and a positive immunostaining was found in the cytoplasm but not in the nucleus. No zonal distribution of glucokinase was observed in livers of such pups. From day 15, at which time a dietary change from milk to laboratory chow begins to take place, glucokinase immunoreactivity increased; this increase was associated with increases in glucokinase activity and in glucokinase protein, and also the immunostaining was observed mainly in the nuclei. At day 21, the glucokinase immunoreactivity was found almost exclusively in the perivenous zone. At day 30, an intense immunostaining was seen both in the perivenous zone and in the periportal zone, being slightly predominant in the former. The present results indicate that dramatic changes in the distribution of glucokinase in developing rat liver may be related to dietary change.

【 授权许可】

Unknown