Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria

FEBS Letters

Iynedjian, Patrick B.²  Hughes, Steven D.¹  Quaade, Christian¹  Newgard, Christopher B.¹  Coats, Ward S.¹  Sestak, Andrea L.¹ 

[1] Center for Diabetes Research, Gifford Laboratories and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA;Division de Biochimie Clinique, University of Geneva School of Medicine, CH-1211 Geneva 4, Switzerland

关键词: Glucokinase;    Islets of Langerhans;    Liver;    Anterior pituitary;    Bacterial expression;   

DOI  :  10.1016/0014-5793(91)80201-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Five variant transcripts of the single rat glucokinase gene have been described that are naturally expressed in islets of Langerhans, liver and anterior pituitary. Four of these were prepared as cDNA and expressed in bacteria in order to begin to address their physiological roles. Expression of constructs pGKB1 (normal islet/pituitary glucokinase) and pGKL1 (normal liver glucokinase) resulted in glucose-dependent, glucokinase-like activity, 7-fold and 45-fold, respectively, above background. Expression of pGKB3 (variant islet/pituitary glucokinase) and pGKL2 (variant liver glucokinase) in contrast, did not result in any glucokinase-like activity.

【 授权许可】

Unknown