Intracellular distribution of hepatic glucokinase and glucokinase regulatory protein during the fasted to refed transition in rats

FEBS Letters

Fernández-Novell, Josep M.¹  Vilaró, Senen²  Castel, Susanna²  Ferrer, Juan C.¹  Bellido, David³  Guinovart, Joan J.¹ 

[1] Departament de Bioquı́mica i Biologia Molecular, Martı́ i Franquès 1, Planta 7, E-08028 Barcelona, Spain;Departament de Biologia Cel.lular, Universitat de Barcelona, E-08028 Barcelona, Spain;Serveis Cientı́fico-tècnics, Universitat de Barcelona, E-08028 Barcelona, Spain

关键词: Glucokinase;    Glucokinase regulatory protein;    Liver;    Refeeding;    Translocation;    GK;    glucokinase;    GKRP;    glucokinase regulatory protein;   

DOI  :  10.1016/S0014-5793(99)01249-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the intracellular distribution in vivo of glucokinase (GK) and glucokinase regulatory protein (GKRP) in livers of fasted and refed rats, using specific antibodies against both proteins and laser confocal fluorescence microscopy. GK was found predominantly in the nucleus of hepatocytes from starved rats. GK was translocated to the cytoplasm in livers of 1- and 2-h refed animals, but returned to the nucleus after 4 h. GKRP concentrated in the hepatocyte nuclei and its distribution did not change upon refeeding. These results show that, in physiological conditions, GKRP is present predominantly in the nuclei of hepatocytes and that the translocation of hepatic GK from and to the nucleus is operative in vivo.

【 授权许可】

Unknown