Mapping the subcellular localization of Fe3O4@TiO2 nanoparticles by X-ray Fluorescence Microscopy

11th International Conference on X-ray Microscopy

Yuan, Y.^1 ; Chen, S.^2 ; Gleber, S.C.^2 ; Lai, B.^2 ; Brister, K.^3 ; Flachenecker, C.^4 ; Wanzer, B.^1 ; Paunesku, T.^1 ; Vogt, S.^2 ; Woloschak, G.E.^1

Department of Radiation Oncology, Northwestern University, Chicago, IL 60611, United States^1

X-ray Sciences Division, Argonne National Laboratory, Argonne, IL 60439, United States^2

Life Sciences Collaborative Access Team, Argonne National Laboratory, Argonne, IL 60439, United States^3

Xradia, Pleasanton, CA 94588, United States^4

关键词: Advanced photon source;    Argonne National Laboratory;    Epidermal growth factor receptors;    Sub-micron resolutions;    Subcellular compartments;    Subcellular distribution;    Subcellular localizations;    X-ray fluorescence microscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012020/pdf
DOI  :  10.1088/1742-6596/463/1/012020

来源: IOP

【 摘 要 】

The targeted delivery of Fe3O4@TiO2nanoparticles to cancer cells is an important step in their development as nanomedicines. We have synthesized nanoparticles that can bind the Epidermal Growth Factor Receptor, a cell surface protein that is overexpressed in many epithelial type cancers. In order to study the subcellular distribution of these nanoparticles, we have utilized the sub-micron resolution of X-ray Fluorescence Microscopy to map the location of Fe3O4@TiO2NPs and other trace metal elements within HeLa cervical cancer cells. Here we demonstrate how the higher resolution of the newly installed Bionanoprobe at the Advanced Photon Source at Argonne National Laboratory can greatly improve our ability to distinguish intracellular nanoparticles and their spatial relationship with subcellular compartments.