Temperature‐induced micellar‐lamellar transformation in binary mixtures of saturated phosphatidylcholines with sodium cholate

FEBS Letters

Polozova, Alla I.¹  Dubachev, Gennady E.¹  Simonova, Tatyana N.¹  Barsukov, Leonid I.¹ 

[1] Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklava 16110, 117871 Moscow V-437, Russian Federation

关键词: Saturated phosphatidylcholine;    Sodium cholate;    Micellar-lamellar transition;    Electron microscopy;    NMR;    Differential scanning calorimetry;    DPPC;    dipalmitoylphosphatidylcholine;    DMPC;    dimyristoylphosphatidylcholine;    ChNa;    sodium cholate;    DSC;    differential scanning calorimetry;    Re;    effective detergent/lipid molar ratio in the mixed structures;   

DOI  :  10.1016/0014-5793(94)01378-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transition states of binary mixtures of dipalmitoyl- and dimyristoylphosphatidylcholines with sodium cholate at the reversible temperature-induced micellar-lamellar transformation were characterized by turbidimetry, electron microscopy, ³¹P NMR and differential scanning calorimetry. This transformation is triggered by the phospholipidacyl chain melting, and appears to include two structural pathways: (i) from discoidal mixed micelles to network-like structures composed of long interlaced rod-like micelles, then to multilayer membrane structures, and finally to multilamellar vesicles; and (ii) from discoidal micelles to membrane fragments and finally to unilamellar vesicles.

【 授权许可】

Unknown