期刊论文详细信息
FEBS Letters
Functional reconstitution of rat ovarian LH/hCG receptor into proteoliposomes
Kolena, Jaroslav1 
[1] Institute of Experimental Endocrinology, Centre of Physiological Sciences, Slovak Academy of Sciences, 833 06 Bratislava, Czechoslovakia
关键词: Reconstitution;    Hormone receptor;    Sodium cholate;    Adenylate cyclase;    (Rat ovary);   
DOI  :  10.1016/0014-5793(89)80769-0
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Rat ovarian membrane LH/hCG receptor was solubilized in various detergents and reconstituted into proteoliposomes. Upon removal of sodium cholate by active absorption on Bio-Beads SM-2, the functional interaction between receptor and adenylate cyclase was restored. Adenylate cyclase was stimulated by hCG, HCG+GTP or GppNHp and NaF. Reconstituted proteoliposomes bound more 125I-hCG (528 fmol/mg protein) than membrane-bound receptors (384 fmol/mg protein). There was no difference, however, in the relative affinity of reconstituted receptor preparations for hCG.

【 授权许可】

Unknown   

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