Functional reconstitution of rat ovarian LH/hCG receptor into proteoliposomes

FEBS Letters

Kolena, Jaroslav¹ 

[1] Institute of Experimental Endocrinology, Centre of Physiological Sciences, Slovak Academy of Sciences, 833 06 Bratislava, Czechoslovakia

关键词: Reconstitution;    Hormone receptor;    Sodium cholate;    Adenylate cyclase;    (Rat ovary);   

DOI  :  10.1016/0014-5793(89)80769-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat ovarian membrane LH/hCG receptor was solubilized in various detergents and reconstituted into proteoliposomes. Upon removal of sodium cholate by active absorption on Bio-Beads SM-2, the functional interaction between receptor and adenylate cyclase was restored. Adenylate cyclase was stimulated by hCG, HCG+GTP or GppNHp and NaF. Reconstituted proteoliposomes bound more ¹²⁵I-hCG (528 fmol/mg protein) than membrane-bound receptors (384 fmol/mg protein). There was no difference, however, in the relative affinity of reconstituted receptor preparations for hCG.

【 授权许可】

Unknown