期刊论文详细信息
FEBS Letters | |
Functional reconstitution of rat ovarian LH/hCG receptor into proteoliposomes | |
Kolena, Jaroslav1  | |
[1] Institute of Experimental Endocrinology, Centre of Physiological Sciences, Slovak Academy of Sciences, 833 06 Bratislava, Czechoslovakia | |
关键词: Reconstitution; Hormone receptor; Sodium cholate; Adenylate cyclase; (Rat ovary); | |
DOI : 10.1016/0014-5793(89)80769-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Rat ovarian membrane LH/hCG receptor was solubilized in various detergents and reconstituted into proteoliposomes. Upon removal of sodium cholate by active absorption on Bio-Beads SM-2, the functional interaction between receptor and adenylate cyclase was restored. Adenylate cyclase was stimulated by hCG, HCG+GTP or GppNHp and NaF. Reconstituted proteoliposomes bound more 125I-hCG (528 fmol/mg protein) than membrane-bound receptors (384 fmol/mg protein). There was no difference, however, in the relative affinity of reconstituted receptor preparations for hCG.
【 授权许可】
Unknown
【 预 览 】
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RO201912020292210ZK.pdf | 228KB | download |