Cloning and characterisation of the human 5‐HT5A serotonin receptor

FEBS Letters

Bull, David¹  Foord, Steven¹  Goodson, Susannah¹  Rees, Stephen¹  Kilpatrick, Gavin¹  den Daas, Izaak¹  Lee, Melanie¹ 

[1] Receptor Biology, Biology Division, Glaxo Research and Development, Park Road, Ware, Herts, SG12 0DP UK

关键词: G protein coupled receptor;    Serotonin;    5-Hydroxytryptamine;   

DOI  :  10.1016/0014-5793(94)01209-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human 5-HT_5A serotonin receptor has been cloned. As with the mouse and rat 5-HT_5A receptors, the gene consists of two coding exons separated by a large intron. The deduced amino acid sequence of the gene reveals a protein of 357 residues which shares 93% (nucleotide) and 84% (amino acid) identity to the cloned mouse 5-HT_5A receptor. We have determined the tissue distribution of the receptor by reverse transcriptase-PCR and found expression in all regions of the brain examined with little or no expression in peripheral tissues. The receptor has been transiently expressed in Cos M6 cells and exhibits a pharmacological profile closely resembling the mouse and rat 5-HT_5A receptors with high, specific binding for ergotamine and methiothepin.

【 授权许可】

Unknown