Cloning and characterisation of the rabbit 5‐HT1Dα and 5‐HT1Dβ receptors

FEBS Letters

Fairweather, Neil²  Lockyer, Michael¹  Giles, Heather¹  Harwood, Gail¹ 

[1] Receptor Pharmacology Research Groups, Wellcome Research Laboratories, Beckenham, Kent BR3 3BS, UK;Molecular Pharmacology Research Groups, Wellcome Research Laboratories, Beckenham, Kent BR3 3BS, UK

关键词: G-protein coupled receptor;    Serotonin;    5-Hydroxytryptamine;    Rabbit;   

DOI  :  10.1016/0014-5793(95)01308-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The genes encoding the rabbit 5HT_1Dα and 5HT_1Dβ receptors have been cloned. The deduced amino acid sequence of these receptors shows 91–92% amino acid sequence identity with their human homologues, and similar high sequence identity with homologues from other species. The receptors were transiently expressed in COS-7 cells and exhibit a pharmacological profile closely resembling their human homologues, including a higher affinity of ketanserin for the 5-HT_1Dα subtype. However, sumatriptan had a lower affinity for both the rabbit receptors compared to their human counterparts. This may be accounted for by differences between the primary amino acid sequences of these species homologues.

【 授权许可】

Unknown