Cloning and characterization of the rat 5‐HT5B receptor

FEBS Letters

Yocca, Frank D.³  Seeburg, Peter H.⁴  Felder, Christian C.²  Nowak, Henry P.³  Grisel, Darcy A.³  Parker, Eric M.³  Wisden, William⁴  Voigt, Mark M.¹  Mahle, Cathy D.³ 

[1] Department of Pharmacology and The Biotechnology Centre, Merville House, University College-Dublin, Belfield, Dublin 4, Ireland;Building 36, Room 3A-15, Laborary of Cell Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA;Department of CNS Biology, Bristol-Myers Squibb Company, 5 Research Parkway, Wallingford, CT 06492, USA;Center for Molecular Biology, University of Heidelberg ZMBH, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

关键词: Serotonin;    5-Hydroxytryptamine;    5-Carboxamidotryptamine;    G-protein-coupled receptor;    Habenula;    CA1 pyramidal cell;    5-HT;    5-hydroxytryptamine;    LSD;    lysergic acid diethylamide;    8-OH-DPAT;    8-hydroxy-2-(di-l-propylamino)tetralin;    5-CT;    5-carboxamidotryptamine;    GTI;    serotonin-O-carboxymethylglycyltyrosinamide;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(93)80368-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene encoding a novel G protein-coupled 5-hydroxytryptamine (5-HT) receptor, termed 5-HT_5B, was cloned. The ligand binding profile of this receptor is distinct from that of other cloned 5-HT receptors. The 5-HT_5B receptor couples to a G protein in COS1 cell membranes; however, activation of the 5-HT_5B receptor does not appear to alter either cAMP accumulation or phosphoinositide turnover in a variety of fibroblast cell lines. In the rat brain, 5-HT_5B gene expression occurs predominantly in the medial habenulae and hippocampal CA1 cells of the adult. Little expression is seen during embryonic development.

【 授权许可】

Unknown