Cloning of an isoform of mouse TGF‐β type II receptor gene

FEBS Letters

Suzuki, Atsushi¹  Shioda, Noriko¹  Ueno, Naoto¹  Maeda, Toshihiko¹  Tada, Masazumi¹ 

[1] Faculty of Pharmaceutical Science, Hokkaido University, Sapporo 060, Japan

关键词: Transforming growth factor-β receptor;    Alternative splicing;    Serine-threonine kinase;    RT-PCR;    reverse transcription-polymerase chain reaction;    TGF-β;    transforming growth factor-β;    TGF-βRII;    TGF-β type II receptor;   

DOI  :  10.1016/0014-5793(94)01156-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A variant of transforming growth factor-β type II receptor (TGF-βRII) cDNA was isolated from a mouse brain cDNA library. The predicted receptor is identical to previously reported mouse TGF-βRII except that the isoform has an insertion sequence of 25 amino acids in the predicted ligand-binding domain. By the use of reverse transcription-polymerase chain reaction (RT-PCR), transcripts for both isoforms were detected in all tissues and developing embryos examined. The isoform transiently expressed in COS cells showed a similar ligand-binding specificity to authentic TGF-βRII. These results suggest that the mouse TGF-βRII gene generates multiple isoforms, possibly by alternative splicing, as reported for activin type IIB receptor; and an isoform which has the extra sequence in the ligand-binding domain is also involved in the TGF-β signal transduction.

【 授权许可】

Unknown