| Genomics | |
| Identification of novel splice variants in horn cancer by RNA-Seq analysis in Zebu cattle | |
| Subhash J. Jakhesara1 Manisha R. Sajnani1 Amrutlal K. Patel1 Chaitanya G. Joshi1 Vaibhav D. Bhatt1 Ajai K. Tripathi1 Prakash G. Koringa1 | |
| 关键词: AS; BLAT; BLAST; HN; HC; NGS; RT-PCR; RT-qPCR; Horn cancer; Transcriptome sequencing; BLAT; Alternative splicing; GT-AG rule; Frame shift; RT-PCR; RT-qPCR; | |
| DOI : 10.1016/j.ygeno.2012.10.001 | |
| 学科分类：医学（综合） | |
| 来源: Academic Press | |
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【 摘 要 】
Horncanceraccountsfornearly83%oftotaltumorsfoundinIndianZebucattle,whichresultsinchronicsufferingandcausesheavyeconomiclosses.Alternativesplicinghasbeenfrequentlyimplicatedinthevarioustypesofcancerprogression.Utilizingthetranscriptomesequencegeneratedbynextgenerationsequencing,weanalyzedthetranscriptdataforthepresenceofalternativesplicingusingBLATprogramandidentified27alternativelysplicedgenes,ofwhich12splicedvariantsappearedtobethenovelsplicedcandidates.Proteinpredictionofthesenovelsplicedvariantsrevealedthatsplicevariationhascausedeithertruncationofprotein,insertion/deletionofstretchofaminoacidsorformationofuniquecarboxyterminus.TheRT-PCRanalysisconfirmedtheexpressionof8ofthe12novelsplicedvariantsobservedbytranscriptomesequencing.Additionally,alteredsplicing/expressionofthesenovelcandidatesbetweencancerandnormaltissuesrevealedbyqPCRsuggeststheirpotentialinvolvementinthedevelopmentofhorncancer.
【 授权许可】
Unknown
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201912090765624ZK.pdf | 716KB |  download |
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