Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen

FEBS Letters

Hino, Katsuhiko¹  Fukuda, Shigeharu¹  Torigoe, Kakuji¹  Kurimoto, Masashi¹  Kurose, Mayumi¹  Taniguchi, Yoshifumi¹  Usui, Mitsuko¹  Namba, Motoshi¹ 

[1] Fujisaki Institute, Hayashibara Biochemical Laboratories Inc., 675-1 Fujisaki, Okayama 702, Japan

关键词: Japanese cedar pollen;    Cry j II;    Cry j I;    Polygalacturonase;    Molecular cloning;    PVDF;    polyvinylidene difluoride;    RT-PCR;    reverse transcription-polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;    PG;    polygalacturonase;    TPCK-trypsin;    N-tosyl-l-phenylalanine chloromethyl ketone treated trypsin;    mAb;    monoclonal antibody;    CNBr;    cyanogen bromide;   

DOI  :  10.1016/0014-5793(94)01022-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cloning of a cDNA from Cry j II, the second major allergen from Japanese cedar (Cryptomeria japonica) pollen, is described. An isolated Cry j II cDNA contained an open reading frame coding for 514 amino acid residues. The mature Cry j II protein consisted of 388 amino acid residues (R⁴⁶—S⁴³³). According to a homology analysis, no amino acid sequence homology was observed between Cry j II and Cry j I, another major allergen. But Cry j II showed homology with polygalacturonase (PG) derived from tomato (40% identity) at the amino acid level. The sequence information can potentially be used to devise an effective course of immunotherapy for Japanese cedar pollinosis.

【 授权许可】

Unknown