Effect of temperature and pH on polygalacturonase production by pectinolytic bacteria Bacillus licheniformis strain GD2a in submerged medium from Raja Nangka (Musa paradisiaca var. formatypica) banana peel waste

International Conference On Food Science and Engineering 2016

Widowati, E.^1 ; Utami, R.^1 ; Mahadjoeno, E.^2 ; Saputro, G.P.^1

Department of Food Science and Technology, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Kentingan Jebres, Surakarta, Central Java

57126, Indonesia^1

Department of Biology, Faculty of Mathematic and Natural Science, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Kentingan Jebres, Surakarta, Central Java

57126, Indonesia^2

关键词: Bacillus licheniformis;    Banana peel wastes;    Effect of temperature;    Optimum conditions;    Pectin concentration;    Polygalacturonase;    Protein concentrations;    Specific activity;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/193/1/012018/pdf
DOI  :  10.1088/1757-899X/193/1/012018

来源: IOP

【 摘 要 】

The aim of this research were to determine the effect of temperature (45°C, 55°C, 65°C) and pH (5.0; 6.0; 7.0) on the increase of total cell count and polygalacturonase enzyme activity produced from raja nangka banana (Musa paradisiaca var. formatypica) peel waste by pectinolytic bacterial Bacillus licheniformis strain GD2a. This research applied two sample repetition and one analysis repetition. The result showed temperature and pH affect total cell count. The total cell count on 45°C and pH 7 recorded the highest number at 9.469 log cell/ml. Temperature and pH also affected pectin concentration at the end of fermentation. The lowest pectin concentration recorded at 45°C and pH 7 was 0.425 %. The highest enzyme activity recorded at 65°C and pH 7 was 0.204 U/ml. The highest enzyme protein concentration was recorded at 65°C and resulted as 0.310 mg/ml on pH 6. The highest specific activity was 19.527 U/mg at 65°C and pH 7. By this result, could be concluded that optimum condition process on polygalacturonase production was at 65°C and pH 7 because it gave highest enzyme activity result (0,204 U/ml).