FEBS Letters | |
ARF1‐regulated phospholipase D in human neutrophils is enhanced by PMA and MgATP | |
Cronin, Paul1  Whatmore, Jacqueline1  Cockcroft, Shamshad1  | |
[1] Department of Physiology, Rockefeller Building, University College London, University Street, London, WC1E 6JJ, UK | |
关键词: Phospholipase D; Neutrophil; ADP ribosylating factor; PMA; MgATP; PLD; phospholipase D; PI 3-kinase; phosphoinositide 3-kinase; PA; phosphatidic acid; PEt; phosphatidylethanol; PMA; phorbol 12-myristate 13-acetate; PKC; protein kinase C; ARF; ADP-ribosylation factor; G-proteins; GTP-binding proteins; fMetLeuPhe; N-formyl-methionyl-leucyl-phenylalanine; GTP-γ-S; guanosine 5′[γ-thio]triphosphate; PLC; inositol lipid-specific phospholipase; PI; phosphatidylinositol; PC; phosphatidylcholine; | |
DOI : 10.1016/0014-5793(94)00930-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Human neutrophil PLD activity stimulated with GTP-γ-S was reconstituted with recombinant ARF1 in cytosol-depleted cells. PMA-pretreatment of intact cells greatly enhanced the subsequent reconstitution of the ARF1-regulated PLD activity. This enhancement was only observed provided that the intact cells were pretreated with PMA, suggesting the stable recruitment of a cytosolic component, presumably protein kinase C, to the membranes. rARF1-reconstituted PLD activity was not dependent on MGATP, but could be considerably enhanced by MgATP. Maximal effects of MgATP were seen at 1 mM. This enhancement by MgATP could not be attributed to protein kinase C. Neomycin was found to inhibit ARF1-regulated PLD activity suggesting the requirement for polyphosphoinositides. We conclude: (i) that many of the observed effects of PMA may be dependent on the presence of the small GTP-binding protein, ARF, and (ii) polyphosphoinositides are required for ARF1-stimulated PLD activity.
【 授权许可】
Unknown
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