Effect of iC3b binding to immune complexes upon the phagocytic response of human neutrophils: synergistic functions between FcγR and CR3

FEBS Letters

Kobayashi, Katsuya¹  Takahashi, Kazuhiko¹  Ohkuro, Masayoshi¹  Nagasawa, Shigeharu¹  Ogura-Masaki, Michiko¹  Sakai, Masatoshi¹ 

[1] Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060, Japan

关键词: Neutrophil;    Phagocytosis;    FcγR;    CR3;    Phospholipase D;    IC;    immune complexes;    iC3b-IC;    iC3b-opsonized IC;    PLD;    phospholipase D;    FITC;    fluorescein isothiocyanate;    FIC;    FITC-labeled IC;    mAb;    monoclonal antibody;   

DOI  :  10.1016/0014-5793(95)01036-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We compared the phagocytosis of immune complexes (IC) and iC3b-opsonized derivatives (iC3b-IC) by human neutrophils. The phagocytosis of iC3b-IC via FcγR and CR3 was much greater than that of IC via FcγR alone. Adding ethanol to the cells decreased iC3b-IC phagocytosis to that of IC, which was not affected by these reagents, suggesting that the enhanced phagocytosis is attributable to CR3-mediated phospholipase D activation. The IC phagocytosis was inhibited more effectively by anti-FcγIIIB, whereas the iC3b-IC phagocytosis was partly inhibited only by anti-FcγRII. The main FcγR might differ in IC and iC3b-IC phagocytosis.

【 授权许可】

Unknown