FEBS Letters | |
Fluoride activates diradylglycerol and Superoxide generation in human neutrophils via PLD/PA phosphohydrolase‐dependent and ‐independent pathways | |
Olson, Susan C.1  Lambeth, J.David1  Tyagi, Shiv Raj1  | |
[1] Department of Biochemistry, Emory University Medical School, Atlanta, GA 30322, USA | |
关键词: Superoxide generation; Diacylglycerol; Diradylglycerol; Phospholipase D; Fluoride; Neutrophil; DAG; 1; 2-diacylglycerol; EAG; 1-O-alkyl; 2-acylglycerol; PA; phosphatidic acid; fMLP; formyl-methionylleucyl-phenylalanine; PI; phosphatidylinositol; ATP; adenosine 5 '-triphosphate; CB; cytochalasin B; PC; phosphatidylcholine; TLC; thin-layer chromatography; PBS; phosphate-buffered saline; PETH; phosphatidylethanol; EtOH; ethanol; GM-CSF; granulocyte/monocyte colony-stimulating factor; PMA; phorbol 12-myristate 13-acetate; | |
DOI : 10.1016/0014-5793(90)80439-P | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In contrast to the rapid, ethanol-inhibited Superoxide generation by the receptor-linked agonist formyl-methionyl-leucyl-phenylalanine (fMLP), fluoride-activated Superoxide generation occurs after a prolonged lag, and as shown herein is relatively ethanol-insensitive. We have investigated fluoride-activation of diradylglycerol generation and phospholipase D activity. Fluoride induces a very large increase in diradylglycerol mass (both 1,2-diacylglycerol (DAG) and 1-O-alkyl,2-acylglycerol (EAG)), with kinetics similar to Superoxide generation. Unlike fMLP-activated diglyceride generation which is completely inhibited by ethanol, that produced by fluoride is only partially (30%) blocked. When the phosphatidylcholine pool is 3H-prelabeled, fluoride activates both [3H]phosphatidic acid (PA) and [3H]diglyceride generation with similar kinetics. Partial inhibition of the production of these species by ethanol was seen, coincident with the appearance of [3H]phosphatidylethanol, indicating phospholipase D-dependent transphosphatidylation had occurred. The data are consistent with the fluoride activation of PA and diglyceride generation by both phospholipase D-dependent and -independent (presumably phospholipase C) mechanisms.
【 授权许可】
Unknown
【 预 览 】
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