Anti‐apoptotic role of phospholipase D in spontaneous and delayed apoptosis of human neutrophils

FEBS Letters

Lee, Min-Jung²  Lee, Sang-Hwa¹  Kwak, Jong-Young²  Park, Myung-Ae²  Jung, Dong-Keun¹ 

[1] Dong-A University Institute of Medical Science, Busan 602-103, South Korea;Department of Biochemistry, Dong-A University College of Medicine, Busan 602-103, South Korea

关键词: Phospholipase D;    Apoptosis;    Neutrophil;    Butanol;    Propranolol;    PLD;    phospholipase D;    LPS;    lipopolysaccharide;    GM-CSF;    granulocyte/macrophage colony-stimulating factor;    PA;    phosphatidic acid;    DAG;    diacylglycerol;    PEth;    phosphatidylethanol;    IL-8;    interleukin-8;    dbcAMP;    dibutyryl-cAMP;    GTPγS;    guanosine 5′-O-(3-thiotriphosphate);   

DOI  :  10.1016/S0014-5793(02)02705-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neutrophil apoptosis is a constitutive process that can be enhanced or delayed by signals induced by various stimuli. We investigated the role of phospholipase D (PLD) in neutrophil apoptosis. The apoptotic rate of neutrophils was found to be increased by 1-butanol and decreased by the exogenous addition of PLD. Moreover, the delay of apoptosis by apoptosis-delaying stimuli such as granulocyte/macrophage colony-stimulating factor or lipopolysaccharide (LPS) was also blocked by 1-butanol. Unstimulated PLD activity in cultured cells for 20 h was higher than that in freshly isolated cells and further increased in cultured cells with LPS. These results suggest that PLD is involved in the up-regulation of neutrophil survival.

【 授权许可】

Unknown