Sodium benzylideneascorbate induces apoptosis in HIV‐replicating U1 cells

FEBS Letters

Yamamoto, Naoki³  Aokia, Kazumasa¹  Shiokawa, Daisuke⁴  Maruta, Hideharu¹  Kochi, Mutsuyuki²  Tanimoto, Yutaka¹  Nakashima, Hideki³  Ni-imi, Ei-ichi¹  Tanuma, Sei-ichi¹  Uchiumi, Fumiaki¹  Hattori, Toshihiro¹ 

[1] Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Shinjuku-ku, Tokyo 162, Japan;Ichijoukai Hospital, Ichikawa-shi, Chiba, Japan;Department of Microbiology, Tokyo Medical and Dental University School of Medicine, Bunkyo-ku, Tokyo, Japan;Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Bunkyo-ku, Tokyo, Japan

关键词: HIV replication;    Apoptosis;    Benzylideneascorbate;    Anti-HIV drug;    SBA;    sodium 5;    6-benzylidene-l-ascorbate;    HIV;    human immunodeficiency virus;    TPA;    12-O-tetradecanoxylphorbol 13-acetate;    NFκB;    nuclear factor kappa B;    LTR;    long terminal repeat;    TNF;    tumor necrosis factor;    PKC;    protein kinase C;    bp;    base pair;   

DOI  :  10.1016/0014-5793(94)00832-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

U1 cells, a subclone of U937 cells chronically infected with human immunodeficiency virus type 1 (HIV-1), produced HIV-1 only in the presence of inducers such as 12-O-tetradecanoxylphorbol 13-acetate (TPA) or tumor necrosis factor (TNF)-α. The expression of HIV-antigen on U1 cells induced by TPA or TNF-α was found to be prevented by sodium 5,6-benzylidene-l-ascorbate (SBA) in a concentration-dependent manner. Treatment of U1 cells with SBA in the presence of inducers resulted in cell death with cell shrinkage, chromatin condensation and DNA fragmentation into nucleosomal oligomers, characteristics of apoptosis. In contrast, SBA had scarcely any apoptotic effect on U1 cells in the absence of inducers. SBA did not also induce apoptosis in parental U937 cells in the presence or absence of inducers. These results suggest that HIV-replicating U1 cells selectively undergo apoptosis on treatment with SBA.

【 授权许可】

Unknown