Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to Gαq and not Gα11

FEBS Letters

Lipinsky, Dafna²  Battey, James F.¹  Shapira, Hagit²  Oron, Yoram²  Way, James¹ 

[1] Laboratory of Biological Chemistry, Developmental Therapeutics Program, Division of Cancer Treatment, National Cancer Institute, USA;Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel

关键词: G-protein;    Neuromedin B receptor;    Gastrin-releasing peptide receptor;    Xenopus oocyte;   

DOI  :  10.1016/0014-5793(94)00570-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

G-proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus G_αq and G_α11 and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. G_αq antisense inhibited responses mediated by neuromedin B receptor (NMB-R) by 74%, though not by gastrin-releasing peptide receptor (GRP-R). G_α11 antisense had little effect on either GRP-R- or NMB-R-mediated responses. This suggests that NMB-R couples to G_αq, and that GRP-R and NMB-R show distinct G-protein coupling preferences in the Xenopus oocyte.

【 授权许可】

Unknown