Evidence for a metabostatic opioid ϰ‐receptor inhibiting pertussis toxin‐sensitive metabotropic glutamate receptor‐currents in Xenopus oocytes

FEBS Letters

Watanabe, Shigeru¹  Ueda, Hiroshi¹  Misu, Yoshimi¹  Miyamae, Takeaki¹  Nobuyuki, Fukushima¹ 

[1] Department of Pharmacology, Yokohama City University School of Medicine, Yokohama 236, Japan

关键词: Opioid receptor;    G-protein;    Xenopus oocyte;   

DOI  :  10.1016/0014-5793(95)01204-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glutamate evoked pertussis toxin-sensitive currents in Xenopus oocytes expressing metabotropic glutamate receptor subtype 1 (mGluR1) and exogenous Gilα. The mGluR1-currents were completely blocked by U-73122, a phospholipase C (PLC) inhibitor and by niflumic acid, a chloride channel blocker. In the oocyte further coinjected with poly(A)⁺ RNA from the guinea pig cerebellum, the mGluR1-currents were inhibited by U-50488H, an opioid κ-agonist, and this inhibition was blocked by norbinaltorphimine, an opioid κ-antagonist. These findings suggest that the mRNA encoding a novel subtype of opioid κ-receptor which inhibits Gi1-PLC-mediated currents is present in guinea pig cerebellar poly(A)⁺ fractions.

【 授权许可】

Unknown