Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

FEBS Letters

Köhl, Jörg¹  Kretzschmar, Titus¹  Kroll, Beate¹  Penner, Lothar¹  Klos, Andreas¹  Rechkemmer, Gerhard²  Emde, Monica¹  Jeromin, Andreas²  Bautsch, Wilfried¹ 

[1] Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Konstanty-Gutschow-Str. 8, 3000 Hannover 61, Germany;Physiologisches Institut, Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 3000 Hannover 1, Germany

关键词: Reconstitution;    C5a receptor;    U937;    Xenopus oocyte;    cRNA;    complementary RNA;    C5aR;    C5a receptor;    rhC5a;    recombinant human C5a;    PCR;    polymerase chain reaction;    fMLP;    N-formyl methionyl-leucyl-phenylalanine;    G-protein;    GTP binding protein;    G1;    subclass or G-proteins originally described to inhibit adenyl cyclase;    HEPES;    N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid];   

DOI  :  10.1016/0014-5793(91)81285-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

【 授权许可】

Unknown