Selective coupling of the human anaphylatoxin C5a receptor and α16 in human kidney 293 cells

FEBS Letters

Buhl, Anne Mette¹  Worthen, G.Scott²  Eisfelder, Bartholomew J.³  Russell, Marijane³  Johnson, Gary L.³ 

[1] Department of Biostructural Chemistry, Institute of Chemistry, Aarhus University, 8000 Aarhus C., Denmark;Department of Medicine National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206, USA;Division of Basic Sciences National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206, USA

关键词: C5a receptor;    Gα16;    Human kidney 293 cell;   

DOI  :  10.1016/0014-5793(93)81464-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The peptide C5a which is generated during the complement cascade is an important chemotactic factor involved in the inflammatory response. The C5a receptor (C5aR) primary sequence suggests that it has a serpentine structure of seven transmembrane domains which is typical of classical G-protein-coupled receptors. To investigate the signal transduction mechanism of C5a we transiently expressed the C5aR in combination with different G-protein a subunits in human kidney 293 cells and measured the PLC activity induced upon C5a stimulation. Cotransfection of C5aR and α₁₆ stimulated PLC while cotransfection of C5aR with either α_q or α₁₂ did not.

【 授权许可】

Unknown