Gα16 protein expression is up‐ and down‐regulated following T‐cell activation: disruption of this regulation impairs activation‐induced cell responses

FEBS Letters

Lippert, Eric²  Baltensperger, Kurt¹  Hermouet, Sylvie²  Jacques, Yannick² 

[1] Pharmakologisches Institut, Universität Bern, Bern, Switzerland;INSERM U463, Groupe ‘Récepteurs et Cytokines’, Institut de Biologie, 9 Quai Moncousu, 44035 Nantes, France

关键词: G protein;    Gα16;    TCR activation;    Human T-lymphocyte;   

DOI  :  10.1016/S0014-5793(97)01308-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of heterotrimeric G proteins in T-cell activation is poorly understood. Here we show that in normal, mature human T-cells, expression of Gα₁₆, the 43 kDa α subunit of G₁₆, varies widely, depending on T-cell activation status. Quiescent blood lymphocytes strongly up-regulate Gα₁₆ after Leuco A stimulation: protein expression of Gα₁₆ is maximal at day 4, then decreases. Consistently, in human T-cell clones, expression of Gα₁₆ is high in the first week following activation and decreases rapidly within the second week. In addition, permanent disruption of regulated Gα₁₆ expression in Jurkat T-cells by stable overexpression of 43 kDa Gα₁₆ inhibited Leuco A-induced interleukin-2 production, CD69 up-regulation and cell apoptosis (by 58%, 46% and 74%, respectively), suggesting that coordinate regulation of Gα₁₆ expression is necessary for optimal activation-induced T-cell responses, and that Gα₁₆ proteins may be involved in the negative regulation of TCR signalling.

【 授权许可】

Unknown