Cloning and characterization of a rabbit ortholog of human Gα16 and mouse Gα15

FEBS Letters

Sarau, Henry M.³  Nuthulaganti, Parvathi²  Testa, Tania T.¹  Feild, John A.²  Foley, James J.³  Ellis, Catherine²  Ames, Robert S.² 

[1] Department of Molecular Biology, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Southern Way, Harlow, Essex, UK;Department of Molecular Biology, SmithKline Beecham Pharmaceuticals, 709 Swedeland Rd, King of Prussia, PA 19406-0939, USA;Department of Pulmonary Pharmacology, SmithKline Beecham Pharmaceuticals, 709 Swedeland Rd, King of Prussia, PA 19406-0939, USA

关键词: G-protein;    Gα16;    Gα15;    C3a receptor;    C5a receptor;    Nociceptin receptor;    Orphanin FQ receptor;   

DOI  :  10.1016/S0014-5793(99)01317-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA was cloned from a rabbit spleen cDNA library which encoded a G-protein α subunit peptide of 374 amino acids, that at the peptide level exhibited 86% and 79% identity with human Gα16 and mouse Gα15, respectively. The rabbit Gα subunit cDNA was subcloned into a mammalian expression vector and transiently co-transfected into HEK-293 cells along with cDNAs encoding the human C3a, C5a, or nociceptin/orphanin FQ receptors. In all three cases the rabbit G α subunit behaved similarly to Gα15 or Gα16 and effectively coupled the transfected receptors to intracellular calcium mobilization pathways. By nucleotide sequence homology and functional activity the rabbit Gα subunit appears to be the ortholog of human Gα16 and mouse Gα15.

【 授权许可】

Unknown