Gα11 and Gαq guaninine nucleotide regulatory proteins differentially modulate the response to thyrotropin‐releasing hormone in Xenopus oocytes

FEBS Letters

Lipinsky, Dafna²  Gershengorn, Marvin C.¹  Oron, Yoram² 

[1] Division of Molecular Medicine, Department of Medicine, Cornell University Medical College and The New York Hospital, New York, NY 10021, USA;Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel

关键词: G-protein;    TRH receptor;    Xenopus oocyte;   

DOI  :  10.1016/0014-5793(92)80775-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Xenopus oocytes that express mouse thyrotropin-releasing hormone receptors (TRH-Rs) after injection if RNA transcribed from TRH-R cDNA respond to THR by a depolarizing current. This response is transducer by activation of phosphoinositide-specific phospholipase C and utilizes an as yet unidentified endogenous guanine nucleotide-binding regulatory (G) protein(s). The α subunit of G₁₁ and G_q have recently been shown to couple receptors to activation of phospholipase C. To determine whether there are functional differences between these proteins, we have co-expressed the TRH-R with either α₁₁ or α_q, α₁₁ potentiated the response to TRH (by 61±16%), while α_q inhibited the response (by 37±9%). The changes in amplitudes were accompanied by inverse changes in response latencies. These data show that α₁₁ and α_q differentially modulate signal transduction in Xenopus oocytes.

【 授权许可】

Unknown