FEBS Letters | |
Gα11 and Gαq guaninine nucleotide regulatory proteins differentially modulate the response to thyrotropin‐releasing hormone in Xenopus oocytes | |
Lipinsky, Dafna2  Gershengorn, Marvin C.1  Oron, Yoram2  | |
[1] Division of Molecular Medicine, Department of Medicine, Cornell University Medical College and The New York Hospital, New York, NY 10021, USA;Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel | |
关键词: G-protein; TRH receptor; Xenopus oocyte; | |
DOI : 10.1016/0014-5793(92)80775-C | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Xenopus oocytes that express mouse thyrotropin-releasing hormone receptors (TRH-Rs) after injection if RNA transcribed from TRH-R cDNA respond to THR by a depolarizing current. This response is transducer by activation of phosphoinositide-specific phospholipase C and utilizes an as yet unidentified endogenous guanine nucleotide-binding regulatory (G) protein(s). The α subunit of G11 and Gq have recently been shown to couple receptors to activation of phospholipase C. To determine whether there are functional differences between these proteins, we have co-expressed the TRH-R with either α11 or αq, α11 potentiated the response to TRH (by 61±16%), while αq inhibited the response (by 37±9%). The changes in amplitudes were accompanied by inverse changes in response latencies. These data show that α11 and αq differentially modulate signal transduction in Xenopus oocytes.
【 授权许可】
Unknown
【 预 览 】
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