The N‐ethylmaleimide‐sensitive fusion protein (NSF) is preferentially expressed in the nervous system

FEBS Letters

Betz, Heinrich¹  O'Connor, Vincent¹  Püschel, Andreas W.¹ 

[1] Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Deutschordenstr. 46, D-60528 Frankfurt/Main, Germany

关键词: Mouse;    Development;    Hippocampus;    NSF;    SNAP;    NSF;    N-ethylmaleimide-sensitive fusion protein;    PCR;    polymerase chain reacton;    SNAP;    soluble NSF attachment protein;    SNARE;    SNAP receptor.;   

DOI  :  10.1016/0014-5793(94)00505-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NSF and SNAPs (soluble NSF attachment proteins), originally identified as cytosolic components of intracellular vesicular transport mechanisms, have recently been implicated in Ca²⁺-triggered neurotransmitter release from synaptic terminals. Here, we have investigated the temporal and spatial expression pattern of the rodent NSF and SNAP genes. A single transcript of 4.5 kb is highly expressed in rat brain, whereas only minor amounts of NSF mRNA are found in liver, kidney, heart, lung and skeletal muscle. In situ hybridisation revealed NSF transcripts as early as embryonic day 10 preferentially in the nervous system of mouse embryos. In the adult brain NSF is widely expressed with particularly high levels in the hippocampus. An identical expression profile was observed for α/β-SNAP. Our data are consistent with a central function of NSF and SNAPs in neurotransmission.

【 授权许可】

Unknown