CA1 pyramidal cell diversity is rooted in the time of neurogenesis

eLife

Marco Bocchio¹  Anas Islah¹  Catherine Lopez¹  Rosa Cossart¹  Yannick Bollmann¹  Davide Cavalieri¹  Alexandra Angelova¹  Agnès Baude¹ 

[1] Aix Marseille Université, Inserm, INMED, Turing Center for Living Systems, Marseille, France;

关键词: Hippocampus;    Development;    Pyramidal cell;    Neuronal diversity;    Mouse;   

DOI  :  10.7554/eLife.69270

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Cellular diversity supports the computational capacity and flexibility of cortical circuits. Accordingly, principal neurons at the CA1 output node of the murine hippocampus are increasingly recognized as a heterogeneous population. Their genes, molecular content, intrinsic morpho-physiology, connectivity, and function seem to segregate along the main anatomical axes of the hippocampus. Since these axes reflect the temporal order of principal cell neurogenesis, we directly examined the relationship between birthdate and CA1 pyramidal neuron diversity, focusing on the ventral hippocampus. We used a genetic fate-mapping approach that allowed tagging three groups of age-matched principal neurons: pioneer, early-, and late-born. Using a combination of neuroanatomy, slice physiology, connectivity tracing, and cFos staining in mice, we show that birthdate is a strong predictor of CA1 principal cell diversity. We unravel a subpopulation of pioneer neurons recruited in familiar environments with remarkable positioning, morpho-physiological features, and connectivity. Therefore, despite the expected plasticity of hippocampal circuits, given their role in learning and memory, the diversity of their main components is also partly determined at the earliest steps of development.

【 授权许可】

CC BY