Binding of plant isoperoxidases to pectin in the presence of calcium

FEBS Letters

Greppin, Hubert¹  Penel, Claude¹ 

[1] Laboratoire de Biochimie et Physiologie Végétales, Université de Genève, place de l'Université 3, 1211 Genève 4, Switzerland

关键词: Hypocotyl;    Cucurbita pepo;    Polygalacturonic acid;    Pectin junction zone;   

DOI  :  10.1016/0014-5793(94)80605-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Some of the isoperoxidases present in an extract from zucchini hypocotyls - one anionic and two cationic - exhibited a Ca²⁺-dependent pelletability which resulted from an interaction with pectins. Endogenous pectins could be replaced by polygalacturonic acid or pectin extracted from citrus, but not by highly esterifled pectin. The interaction between the isoperoxidases and the polysaccharides has been studied by centrifugation, by gel filtration, and with pectins attached in wells of a microtitration plate. These various binding tests have shown that the isoperoxidases had an affinity for the pectins in their Ca²⁺-induced conformation.

【 授权许可】

Unknown