cDNAs sequences encoding cytochrome P450 (CYP71 family) from eggplant seedlings

FEBS Letters

Ishizaki-Nishizawa, Osamu¹  Toguri, Toshihiro¹  Kobayashi, Osamu¹  Umemoto, Naoyuki¹ 

[1] Central Laboratory for Key Technology, Kirin Brewery Co., 1-13-5 Fukuura Kanazawa-ku, Yokohama 236, Japan

关键词: Cytochrome P450;    cDNA sequence;    Hypocotyl;    Solanum melongena;    Petunia hybrida;    cDNA;    DNA complementary to RNA;    SDS;    sodium dodecyl sulfate;    SSC;    0.15 M NaCl;    0.015 M Na3 citrate pH 7.0;    SSPE;    0.15 M NaCl;    10 mM Na phosphate;    1 mM EDTA pH 7.4;   

DOI  :  10.1016/0014-5793(93)80266-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three cytochrome P450 (P450) cDNAs were isolated from an eggplant hypocotyl cDNA library using eggplant CYP75 cDNA as a probe. These cDNAs have greater than 65% identity in their amino acid sequences, indicating that they belong to the same family. Comparison of these with P450 proteins from other sources showed that the protein with the greatest degree of homology is CYP71, isolated from avocado fruits (~ 48%). We concluded that they are novel members of the CYP71 gene family (CYP71A2, 3 and 4). We have examined the level of mRNA transcripts from the CYP71 family in eggplant hypocotyl tissues and petunia flower buds, and found that the level of transcripts is developmentally regulated in the flower bud.

【 授权许可】

Unknown