Cloning of eggplant hypocotyl cDNAs encoding cytochromes P450 belonging to a novel family (CYP77)

FEBS Letters

Toguri, Toshihiro¹  Tokugawa, Klazuhisa¹ 

[1] Central Laboratory for Key Technology, Kirin Brewery Co. Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama 236, Japan

关键词: Cytochrome P450;    cDNA sequence;    Hypocotyl;    Solanum melongena;    P450;    cytochrome P450;    PCR;    polymerase chain reaction;    SSC;    0.15 M NaCl/0.015 M Na3-citrate pH 7.0;    SSPE;    0.15 M NaCl/10 mM Na-phosphate/1 mM EDTA pH 7.4;   

DOI  :  10.1016/0014-5793(94)80286-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

From eggplant hypocotyl tissues we have cloned two closely related cDNAs encoding cytochromes P450 (P450s) by PCR amplification using a primer designed based on the highly conserved sequence among the known eggplant P450s. One cDNA lacks the NH₂-terminal short sequence that is present in the other, full-length cDNA. The two predicted protein sequences are 71% identical with each other and show less than 30% identity with any other known P450s. It is concluded that these P450s, which are termed CYP77A1 and -A2, belong to a hitherto unknown P450 family.

【 授权许可】

Unknown