期刊论文详细信息
Journal of Applied Biological Sciences | |
Effects of Plant Growth Regulators on Callus Formation in Different Explant of Calendula officinalis L. | |
Nüket AKANIL BİNGÖL1  Burcu ÇETİN1  Betül KURTULUŞ1  | |
关键词: Calendula officinalis L.; Cotyledon; Cotyledon node; Hypocotyl; Plant growth regulators.; | |
DOI : | |
学科分类:生物科学(综合) | |
来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente | |
【 摘 要 】
ThisstudyhasbeenconductedtoinvestigatedifferentexplantsofplantgrowthregulatorsoncallusinductionofCalendulaofficinalisL.medicinalandaromaticherb.Theseedsweresurfacesterilizedusing70%ethanolfor3minutes,10%commercialbleachfor5minutesandrinsed3timeswithsterilewaterfor3minutes.TheseedsweregerminatedinMurashigeandSkoog(MS)medium.Hypocotyl,cotyledonandcotyledonnodeexplantshavebeenexcisedfromtheplantletsobtainedfrominvitrogerminatedseeds.ThreeexplantswereculturedonMSmediasupplementedwithvariousconcentrationsofcytokinin(BAP;1.0,2.0mgL-1)andauxin(IBA;0.1,0,5mgL-1)forcallusinduction.Attheendofeightweeks,thebestresultswereobservedinatreatmentwith1mgL-1BAP+0.5mgL-1IBAand2mgL-1BAP+0.5mgL-1IBA100%onthehypocotyl;1mgL-1BAP+0.5mgL-1IBAand2mgL-1BAP+0.5mgL-1IBA88%onthecotyledon;2mgL-1BAP+0.1mgL-1IBA100%onthecotyledonnodes.Thedataobtainedfromthepathisinstructivetogenetransformation,phytoremediationandcellculturestudy.【 授权许可】
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Files | Size | Format | View |
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RO201912090818749ZK.pdf | 249KB | download |