Elongation Inhibition, Stem Enlargement, and Histological Stem Changes Caused by Vertical Load in Several Epigeal Leguminous Crops

Japanese Journal of Crop Science

Jun INOUYE¹  Il-Doo JIN¹  Shao-Hui ZHENG¹ 

关键词: Emergence;    荷重;    Hypocotyl;    下胚軸;    Leguminous crops;    出芽;    Seedlings;    マメ科作物;    Vertical load.;    芽ばえ.;   

DOI  :  10.1626/jcs.62.502

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

Eight epigeal leguminous crops were used as materials : Soybean (Glycine max Merr.), kidney bean (Phaseolus vulgaris L.), hyacinth bean (Lablab purpureus Sweet.), cowpea (Vigna unguiculata Walp.), mung bean (V. radiata R. Wilczek), black gram (V. mungo Hepper), moth bean (V. aconitifolius Marechal), and peanut (Aracis hypogaea L.). When seedlings had grown to 3 cm in height in soil with 13% moisture by volume at 25°C, covering soil was removed and marks with india ink were put on the seedlings at 2-mm intervals from the top of plumular hooks downward. After the seedlings were covered with soil again up to the top of plumules, a stopper, for vertical load treatment, was placed on the top of each plumule. These procedures were performed quickly under dim green light to minimize confounding factors. Forty-eight hours after the beginning of treatment, hypocotyl length and stem thickness were measured, and the stems were examined histologically (longitudinal and cross-sectional) using a microtome technique. In all crops tested, hypocotyl elongation was inhibited by vertical load treatment, and the inhibition was the largest from the top of the plumular hook down 4 mm in each seedling. Elongation inhibition was the greatest (ca. 66%) in the cowpea and the smallest (ca. 21%) in the peanut. Furthermore, in each seedling whose elongation was inhibited, stem thickness increased. Stem thickness was found to correlate positively with elongation inhibition in each crop (r=0.621*∼0.907***). The results suggest that elongation inhibition and stem enlargement of seedlings may be caused by elongation inhibition and enlargement of cells in the cortex and in pith tissues of seedlings.

【 授权许可】

Unknown