Calcium influx blocks the skeletal muscle acetylcholine receptor α‐subunit gene in vivo

FEBS Letters

Huang, Chang-Fen¹  Schmidt, Jakob¹ 

[1] Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA

关键词: Skeletal muscle;    Acetylcholine receptor;    Regulation;    Calcium;    Excitation-transcription coupling;    Gallus domesticus;    AChR;    acetylcholine receptor;    ECC;    excitation-contraction coupling;   

DOI  :  10.1016/0014-5793(94)80283-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transcriptional activity of the acetylcholine receptor α-subunit gene was measured in denervated chick skeletal muscle in response to calcium-active drugs, using a ribonuclease protection version of the conventional run-off assay. The L-channel agonist (−)Bay-K6844 and the calcium ionophore A23187 mimicked, and the intracellular chelator BAPTA and the calcium channel blockers D600 and nifedipine blocked, the effect of electrostimulation. These results suggest that influx of extracellular calcium is an integral component of the membrane depolarization-receptor gene inactivation cascade.

【 授权许可】

Unknown