Expression of myogenic factors in skeletal muscle and electric organ of Torpedo californica

FEBS Letters

Neville, Craig M.¹  Schmidt, Jakob¹ 

[1]Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA

关键词: Myogenic factor;    Electric organ;    Skeletal muscle;    Regulation;    Acetylcholine receptor;    Torpedo californica;    AChR;    acetylcholine receptor;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(92)80647-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fish electric organ is a skeletal muscle homolog in which many muscle-specific genes are inhibited while acetylcholine receptor is expressed at high levels. The molecular mechanisms underlying this discoordinate regulation have not yet been explored. We have obtained partial sequences for MyoD, myogenin, and myf5 from Torpedo californica and have measured their mRNAs in several organs, using ribonuclease protection. We have found that MyoD and myf5 are expressed at comparable levels in muscle and electric organ, whereas myogenin transcripts could not be detected in either tissue. Acetylcholine receptor α subunit mRNA, on the other hand, is two orders or magnitude more abundant in electric tissue. We conclude that neither the loss of contractile proteins from, nor the enhanced expression of acetylcholine receptor genes in, the differentiating electrocyte is a simple consequence of the abundance of myogenic factor messages.

【 授权许可】

Unknown