Non‐uniform distribution of phospholipids in (Na+ + K+‐ATPase‐rich membranes from Torpedo marmorata electric organ evidenced by spin—spin interactions between spin‐labeled phospholipids

FEBS Letters

Zachowski, Alain¹  Devaux, Philippe F.¹ 

[1] Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France

关键词: Na+ + K+)-ATPase;    Electric organ;    Lipid-protein interaction;    Spin labeling;    Electron spin resonance;    T. marmorata;   

DOI  :  10.1016/0014-5793(83)80828-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

(Na⁺ + K⁺)-ATPase membranes from Torpedo marmorata electric organ were labeled with high concentrations of paramagnetic phospholipid analogs (up to 5 mol of spin labels for 100 mol of endogeneous phospholipids). The mobile lipid bilayer component of the complex resonance spectra obtained showed low-field linewidth broadening due to spin—spin interactions; this was used as a measure of probe concentration in this compartment. Our results show that in the lipid shell surrounding the protein, there is a considerable enrichment in negatively charged phospholipids over neutral ones.

【 授权许可】

Unknown