Monomeric and dimeric forms of cholesterol esterase from Candida cylindracea

FEBS Letters

Ghosh, Debashis¹  Erman, Mary¹  Duax, William L.¹  Kaiser, Rudolf²  Jörnvall, Hans² 

[1] Medical Foundation of Buffalo Inc., 73 High Street, Buffalo, NY 14203-1196, USA;Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden

关键词: Cholesterol esterase;    Primary structure;    Quaternary structure;    Fingerprint analysis;    Microheterogeneity;   

DOI  :  10.1016/0014-5793(94)80257-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cholesterol esterase from Candida cylindracea was separated into two fractions, corresponding to a dimeric and a monomeric form. Fingerprint analysis after lysine cleavages shows identical patterns, suggesting lack of primary differences. Crystals obtained from the two proteins differ and suggest the possibility of an equilibrium between the two forms, influenced by the substrate cholesterol linoleate, which appears to stabilize the more active, dimeric form. All crystals have dimers as the asymmetric unit. The primary structure of the enzyme was determined at the peptide level and shows only one difference, Leu-350 instead of Ile, from a DNA-deduced amino acid sequence, and conservation of features typical for cholesterol esterases characterized.

【 授权许可】

Unknown