TMB colorimetric cholesterol biosensor based on reduced graphene oxide-Hemin nanocomposites

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Zeng, Junxiang^1 ; Tang, Zijian^1 ; Li, Guiyin^1

China School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi

541004, China^1

关键词: Cholesterol biosensor;    Cholesterol esterase;    Cholesterol oxidase;    Clinical diagnosis;    Correlation coefficient;    Reduced graphene oxides;    Regression equation;    Spectrophotometric determination;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/2/022134/pdf
DOI  :  10.1088/1755-1315/252/2/022134

来源: IOP

【 摘 要 】

Cholesterol level is an important reference index in clinical diagnosis. Herein, a highly sensitive TMB colorimetric cholesterol biosensor was constructed based on the good catalytic activity of reduced graphene oxide-Hemin nanocomposites (HGNs) for H2O2 and the color rendering of TMB. Cholesterol is decomposed into H2O2 catalysed by cholesterol oxidase and cholesterol esterase. Colorless TMB can produce blue cation roots when HGNs catalyzes H2O2 reduction. The content of cholesterol can be obtained by spectrophotometric determination of the absorbance of the solution. In the range of 0-100 μ g/mL, the absorbance is linearly related to the cholesterol concentration, the regression equation was Y=0.000629X+0.02876 with a correlation coefficient of 0.99395. The sensor also has a great recovery rate (100.4-101.6%) and RSD (1.78-2.15%) in the detection of human serum samples. This indicated that TMB colorimetric cholesterol biosensor has great potential in clinical diagnosis.