High performance thin layer chromatography fingerprint analysis of guava (Psidium guajava) leaves

International Symposium on Bioinformatics, Chemometrics and Metabolomics

生物科学;化学

Astuti, M.^1 ; Darusman, L.K.^1,2 ; Rafi, M.^1,2

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Dramaga, West Java, Bogor, Indonesia^1

Tropical Biopharmaca Research Center, Bogor Agricultural University, Taman Kencana, West Java, Bogor, Indonesia^2

关键词: Derivatizations;    Fingerprint analysis;    Gallic acids;    High performance thin layer chromatography;    Medicinal plants;    Mobile phase;    Psidium guajava;    Validation criteria;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/835/1/012018/pdf
DOI  :  10.1088/1742-6596/835/1/012018

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

High-performance thin layer chromatography (HPTLC) fingerprint analysis is commonly used for quality control of medicinal plants in term of identification and authentication. In this study, we have been developed HPTLC fingerprint analysis for identification of guava (Psidium guajava) leaves raw material. A mixture of chloroform, acetone, and formic acid in the ratio 10:2:1 was used as the optimum mobile phase in HPTLC silica plate and with 13 bands were detected. As reference marker we chose gallic acid (Rf= 0.21) and catechin (Rf= 0.11). The two compound were detected as pale black bands at 366 nm after derivatization with sulfuric acid 10% v/v (in methanol) reagent. Validation of the method was met within validation criteria, so the developed method could be used for quality control of guava leaves.