Stimulation of prostaglandin production by hepatocyte growth factor in human gastric carcinoma cells

FEBS Letters

Miyazawa, Keiji¹  Kitamura, Naomi¹  Shibamoto, Sayumi²  Hayakawa, Makio²  Ito, Fumiaki²  Takeuchi, Kenji²  Hori, Takamitsu²  Oku, Naoto³ 

[1] Institute for Liver Research, Kansai Medical University, Moriguchi, Osaka 570, Japan;Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573-01, Japan;Department of Radiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Shizuoka 422, Japan

关键词: Hepatocyte growth factor;    Epidermal growth factor;    Prostaglandin;    Arachidonic acid;    Phospholipase A2;    Human gastric carcinoma cell;   

DOI  :  10.1016/0014-5793(93)80705-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatocyte growth factor (HGF), a protein with pleiotropic biological activity affecting cell growth and motility, was found to markedly activate prostaglandin production in human gastric carcinoma TMK-1 cells. HPLC analysis revealed that HGF stimulated the production of prostaglandin E₂ (PGE₂), which is the major prostaglandin produced in these cells. HGF maximally stimulated PGE₂ production at a concentration of 10 , and it was a more potent stimulator of PGE₂ production than epidermal growth factor (EGF), which is known to stimulate prostaglandin production in various cell lines. The simultaneous addition of HGF and EGF caused no further stimulation of the PGE₂ production observed in HGF-treated cells. We showed also that HGF increased the arachidonate release from TMK-1 cells, which release was completely suppressed by the addition of phospholipase A₂ (PLA₂) inhibitors. Further studies in vitro showed that HGF enhanced cellular activities of cytosolic PLA₂ and cyclooxygenase 1.5-fold each. These results indicate that HGF stimulates prostaglandin production through increases in both cytosolic PLA₂ and cyclooxygenase activities.

【 授权许可】

Unknown