FEBS Letters | |
Epidermal growth factor (EGF), tumor promoter 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium and stimulate arachidonic acid release and PGE2/6‐keto PGF1α production in cultured porcine thyroid cells | |
Shimizu, Yoshifusa1  Yamada, Takashi1  Takasu, Nobuyuki1  | |
[1] Department of Gerontology, Endocrinology and Metabolism, School of Medicine, Shinshu University, Asahimachi 3-1-1, Eatsumoto, Nagano-ken 390, Japan | |
关键词: Epidermal growth factor; 12-O-Tetradecanoyl 13-acetate; Ionophore A23187; Ca2+; Fura-2; Prostaglandin; (Thyroid); | |
DOI : 10.1016/0014-5793(87)81128-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Epidermal growth factor (EGF), 12-O-tetradecanoylphorbol 13-acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium ([Ca2+]i) and stimulate arachidonic acid release and production of PGE2 and 6-keto PGF1α an end metabolite of PGI2, in cultured porcine thyroid cells. Addition of EGF, TPA or A23187 to the cells loaded with fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i which is the earliest event after mitogen stimulation. This [Ca2+]i response occurs immediately, reaching a maximum within several seconds. EGF, TPA and A23187 stimulate arachidonic acid release and PGE2 and 6-keto PGF1α production; the maximum effects are obtained after 2–4 h incubation. EGF, TPA and A23187 increase [Ca2+]i and then stimulate arachidonic acid release and PG production.
【 授权许可】
Unknown
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