Epidermal growth factor (EGF), tumor promoter 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium and stimulate arachidonic acid release and PGE2/6‐keto PGF1α production in cultured porcine thyroid cells

FEBS Letters

Shimizu, Yoshifusa¹  Yamada, Takashi¹  Takasu, Nobuyuki¹ 

[1] Department of Gerontology, Endocrinology and Metabolism, School of Medicine, Shinshu University, Asahimachi 3-1-1, Eatsumoto, Nagano-ken 390, Japan

关键词: Epidermal growth factor;    12-O-Tetradecanoyl 13-acetate;    Ionophore A23187;    Ca2+;    Fura-2;    Prostaglandin;    (Thyroid);   

DOI  :  10.1016/0014-5793(87)81128-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Epidermal growth factor (EGF), 12-O-tetradecanoylphorbol 13-acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium ([Ca²⁺]_i) and stimulate arachidonic acid release and production of PGE₂ and 6-keto PGF_1α an end metabolite of PGI₂, in cultured porcine thyroid cells. Addition of EGF, TPA or A23187 to the cells loaded with fura-2, a fluorescent Ca²⁺ indicator, causes an immediate increase in [Ca²⁺]_i which is the earliest event after mitogen stimulation. This [Ca²⁺]_i response occurs immediately, reaching a maximum within several seconds. EGF, TPA and A23187 stimulate arachidonic acid release and PGE₂ and 6-keto PGF_1α production; the maximum effects are obtained after 2–4 h incubation. EGF, TPA and A23187 increase [Ca²⁺]_i and then stimulate arachidonic acid release and PG production.

【 授权许可】

Unknown