Effect of phenylephrine on pyruvate dehydrogenase activity in rat hepatocytes and its interaction with insulin and glucagon

FEBS Letters

McCormack, J.G.¹  Assimacopoulos-Jeannet, F.²  Jeanrenaud, B.² 

[1] Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, England;Laboratoires de Recherches Métabolique, University of Geneva Medical School, 64, Avenue de la Roseraie, 1211 Geneva 4, Switzerland

关键词: (Liver);    Pyruvate dehydrogenase;    α1-Adrenergic receptor;    Glucagon;    Ca2+;    Ionophore A23187;   

DOI  :  10.1016/0014-5793(83)80421-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In isolated rat hepatocytes phenylephrine promotes a rapid increase in the amount of pyruvate dehydrogenase present in its active form (PDH_a). This action is mediated by α₁-adrenergic receptors and is not observed in Ca²⁺-depleted hepatocytes. It is mimicked by the Ca²⁺ ionophore A23187. No changes in metabolites known to affect PDH activity are measured 3 min after addition of phenylephrine. Glucagon also increases PDH_a, its action is additive to that of phenylephrine. The action of phenylephrine on PDH_a could be explained by an increase in mitochondrial free Ca²⁺.

【 授权许可】

Unknown