Over‐expression of protein kinase C‐α enhances platelet‐derived growth factor‐ and phorbol ester‐ but not calcium ionophore‐induced formation of prostaglandins in NIH 3T3 fibroblasts

FEBS Letters

Totzke, Frank²  Dieter, Peter²  Finkenzeller, Günter¹  Marmé, Dieter²  Fitzke, Edith² 

[1] Medizinische Universitätsklinik, Hugstetter Strasse 55, Germany;Institut für Molekulare Zellbiologie, Mooswaldallee 1-9 Germany

关键词: Over-expression;    Protein kinase C;    Ca2+ release;    Prostaglandin;    Arachidonic acid;    PKC;    protein kinase C;    AA;    arachidonic acid;    PG;    prostaglandin;    hPDGF;    human recombinant platelet-derived growth factor;    Fura-2/AM;    Fura-2 acetoxymethyl ester;    HPLC;    high-performance liquid chromatography;    [Ca2+]i;    intracellular concentration of free Ca2+;    PMA;    phorbol 12-myristate 13-acetate;   

DOI  :  10.1016/0014-5793(93)80610-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Over-expression of human protein kinase C-α in murine NIH 3T3 fibroblasts is associated with an increased platelet-derived growth factor- and phorbol ester-mediated formation of prostaglandins, whereas the calcium ionophore-induced release of arachidonic acid metabolites is unaffected; however, the differences of arachidonic acid and prostaglandin formation are much more pronounced with platelet-derived growth factor than with phorbol ester. Platelet-derived growth factor induces an identical elevation of intracellular free calcium in control and protein kinase C-α over-expressing cells; the phorbol ester has no effect on intracellular free calcium in both cell lines. These results demonstrate that protein kinase C-α may couple to arachidonic acid cascade in NIH 3T3 fibroblasts.

【 授权许可】

Unknown