Promoters of epithelialization induce expression of vascular endothelial growth factor in human gastric epithelial cells in primary culture

FEBS Letters

Ogura, Keiji³  Maeda, Shin³  Terano, Akira¹  Takahashi, Morio³  Mikami, Yasuyo⁴  Omata, Masao³  Mafune, Ken-ichi²  Mori, Kiyoshi² 

[1] Second Department of Medicine, School of Medicine, Dokkyo University, Mibu, Tochigi 321-02, Japan;Second Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan;Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan;Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan

关键词: Vascular endothelial growth factor;    Hepatocyte growth factor;    Epidermal growth factor;    Gastric epithelial cells;    Primary culture;    VEGF;    vascular endothelial growth factor;    VPF;    vascular permeability factor;    HGF;    hepatocyte growth factor;    EGF;    epidermal growth factor;    BME;    basal Eagle's medium;    MEM;    minimal essential medium;    HEPES;    N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid;    BSA;    bovine serum albumin;    HBSS;    Hanks' balanced salt solution;    EDTA;    ethylenediaminetetraacetic acid;    FBS;    fetal bovine serum;    RT-PCR;    reverse transcription polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(97)01354-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Both epithelialization and angiogenesis are indispensable processes in gastric ulcer healing. Coordination between these processes has not been well studied. In the present study, we have established a new primary culture system of human gastric epithelial cells and investigated the effect of epithelialization stimulants on a specific angiogenic factor, vascular endothelial growth characterized as epithelial cells. Both epithelialization stimulants, hepatocyte growth factor (HGF) and epidermal growth factor (EGF), significantly stimulated vascular EGF expression in gastric epithelial cells. HGF and EGF receptors were expressed by the cells, suggesting that regulation may be mediated through specific receptors.

【 授权许可】

Unknown