Molecular cloning of crustacean putative molt‐inhibiting hormone (MIH) precursor

FEBS Letters

Keller, Rainer¹  Mangerich, Sigrid¹  Weidemann, Wolfgang M.¹  Klein, Jörg M.¹  de Kleijn, Dominique P.V.² 

[1] Institute for Zoophysiology, University of Bonn, Endenicher Allee 11-13, D-53115 Bonn, Germany;Zoological Laboratory, Faculty of Sciences, Catholic University, Toernoiveld, 6535 ED Nijmegen, The Netherlands

关键词: Molt-inhibiting hormone;    Neuropeptide precursor;    cDNA sequence;    Crustacea;    CHH;    crustacean hyperglycemic hormone;    CPRP;    CHH precursor related peptide;    GIH (=VIH);    gonad-inhibiting hormone (vitellogenesis-inhibiting hormone);    MIH;    molt-inhibiting hormone;    MTXO;    medulla terminalis X-organ;    PCR;    polymerase chain reaction;    SG;    sinus gland;    SP;    signal peptide;   

DOI  :  10.1016/0014-5793(93)81699-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding the complete precursor of the putative molt-inhibiting hormone (MIH) of the shore crab, Carcinus maenas, was isolated and sequenced. The precursor consists of a putative 35 amino acid signal peptide and the 78 amino acid mature MIH. The deduced MIH amino acid sequence is in complete agreement with the sequence previously determined by Edman degradation. In situ hybridization revealed MIH-expression in a subpopulation of large neurosecretory perikarya of the medulla terminalis X-organ in the eyestalk.

【 授权许可】

Unknown