Identification of N‐ and C‐terminal corticotropin peptides in the M r 80 000 form of neurophysin

FEBS Letters

Garnier, D.¹  Morel, A.¹  Beguin, P.¹  Cohen, P.¹ 

[1] Groupe de Neurobiochimie Cellulaire et Moléculaire, Université Pierre et Marie Curie, 96 boulevard Raspail, 75006 Paris, France

关键词: Corticotropin;    Neurophysin;    Neuropeptide precursor;    High-performance liquid chromatography;    Tryptic peptide mapping;    Hypothalamus;    SDS;    sodium dodecyl sulfate;    CPB;    carboxypeptidase B;    DFP;    diisopropylfluorophosphate;    ACTH;    adrenocorticotropin hormone;    HPLC;    high-performance liquid chromatography;    IEF;    isoelectric focusing;    pI;    isoelectric point;   

DOI  :  10.1016/0014-5793(82)81024-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ¹²⁵I-labeled M _r 80 000 form of neurophysin has been purified from bovine neurohypophysi. Tryptic digests of this species were analyzed, prior to or after treatment with carboxypeptidase B, by high-pressure liquid chromatography followed by isoelectric focusing and the fragments compared with those generated by a similar treatment of reference bovine 1–39 adrenocorticotropin. The ACTH peptides 22–39 and 1–8, as well as the 1–7 derivative of the latter were identified by those two independent criteria. This provides chemical evidence supporting the hypothesis [8] that high M _r neurophysin may contain the sequence of ACTH.

【 授权许可】

Unknown