Molecular cloning of two distinct vasotocin precursor cDNAs from chum salmon (Oncorhynchus keta) suggests an ancient gene duplication

FEBS Letters

Coe, Imogen R.²  Morley, Steven D.¹  Richter, Dietmar¹  Sherwood, Nancy M.²  Mahlmann, Stefan¹  Heierhorst, Jörg¹ 

[1] Institut für Zellbiochemie und Klinische Neurobiologie, UKE, Universität Hamburg, Martinistr. 52, 2000 Hamburg 20, FRG;Department of Biology, University of Victoria, Victoria, BC, Canada v8w 2Y2

关键词: Vasotocin;    Neurophysin;    Neuropeptide;    Gene duplication;    Teleost fish;    VT;    vasotocin;    NP;    neurophysin;    ar;    amino acid residue(s);    nr;    nucleotide residue(s);    SSPE;    0.15 M sodium chloride;    10 mM sodium phosphate (pH 7.4) 1 mM ethylenediaminetetraacetate;   

DOI  :  10.1016/0014-5793(90)80129-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structures of two different vasotocin precursors from chum salmon brain have been elucidated through the molecular cloning of their corresponding cDNAs. Although the predicted precursors, consisting respectively of 153 and 158 amino acids, have the same structural organisation, they show 35% amino acid sequence divergence, of which only approximately half are isofunctional substitutions. Remarkably, while the C terminal segments of both precursors resemble the glycopeptide moiety of the related mammalian vasopressin precursor, both salmon precursors lack consensus sequences for N-glycosylation.

【 授权许可】

Unknown